Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1982-9-10
pubmed:abstractText
An in vitro system using nuclei from parvovirus H-1-infected cells was used to characterize the influence of inhibitors of mammalian DNA polymerases on viral DNA synthesis. The experiments tested the effects of aphidicolin, which is highly specific for DNA polymerase alpha, and 2',3'-dideoxythymidine-5'-triphosphate (ddTTP), which inhibits cellular DNA polymerases in the order gamma greater than beta greater than alpha. Both aphidicolin and ddTTP were inhibitory, indicating that both polymerase alpha and a ddttp-sensitive enzyme are required for viral DNA synthesis. This was seen more clearly in kinetic measurements, which indicated an initial period of rapid DNA synthesis with the participation of polymerase alpha, followed by a period of less rapid, but more sustained, rate of DNA synthesis carried out by a ddTTP-sensitive enzyme, probably polymerase gamma. One interpretation of the results is that polymerase alpha functions in a strand displacement stage of the viral DNA replication mechanism, whereas polymerase gamma serves to convert the displaced single strands back to double-strand replicative form.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-1206707, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-196758, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-205546, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-221504, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-226127, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-270687, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-289453, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-293659, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-354497, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-357012, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-38782, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-440966, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-450707, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-482131, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-4855740, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-5166448, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-597856, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-6156541, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-6160581, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-6246822, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-6250448, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-643610, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-673840, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-6777759, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-6782551, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-692726, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-6940154, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-6947222, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-7236628, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-723940, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-789912, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-835240, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-867815, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-914852, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-916022, http://linkedlifedata.com/resource/pubmed/commentcorrection/6808155-967244
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
982-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
DNA polymerase requirements for parvovirus H-1 DNA replication in vitro.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't