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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1982-6-24
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pubmed:abstractText |
A methyltransferase that methylates one of the proteins involved in chemotactic adaptation to sensory stimuli in Bacillus subtilis was purified to homogeneity. The enzyme utilizes S-adenosylmethionine as donor for a methyl group that is transferred to a glutamate residue in a 69 000-mol.wt. membrane protein and also to a protein of 19 000 mol.wt. The molecular weights of the denatured enzyme by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and of the native enzyme by gel-filtration chromatography both show the protein to be a 44 000-mol.wt. monomer. Isoelectric focusing of the purified methyltransferase showed the protein to be a single species with isoelectric point pI 5.4. On the basis of a molecular weight of 44 000, the molar absorption coefficient at 262 nm of the enzyme is 10.9 x 10(4) M-1 . cm-1. The Km of the enzyme for S-adenosylmethionine is about 2 microM. The Ki for S-adenosylhomocysteine is about 0.2 microM. Ca2+ is a competitive inhibitor of methylation, with a Ki of 0.065 microM. The enzyme methylates membranes from the wild-type more efficiently than membranes isolated from a mutant strain defective in chemotaxis. The enzyme is unable to methylate Escherichia coli membranes.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-1105570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-130824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-13907377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-16888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-322131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-323250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-323255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-333433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-333434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-337305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-403829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-412110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-4134806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-4326772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-4373740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-4560688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-4563019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-4850305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-4916925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-5032346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-5349286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-5432796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-5438363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-634372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-68686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-708712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-7356615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-808854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-926803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6803772-965375
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
199
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
795-805
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:6803772-Bacillus subtilis,
pubmed-meshheading:6803772-Chemotaxis,
pubmed-meshheading:6803772-Chromatography, Affinity,
pubmed-meshheading:6803772-Chromatography, Gel,
pubmed-meshheading:6803772-Kinetics,
pubmed-meshheading:6803772-Methylation,
pubmed-meshheading:6803772-Molecular Weight,
pubmed-meshheading:6803772-Protein Methyltransferases,
pubmed-meshheading:6803772-Protein-Arginine N-Methyltransferases
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pubmed:year |
1981
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pubmed:articleTitle |
Purification and characterization of methyl-accepting chemotaxis protein methyltransferase I in Bacillus subtilis.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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