Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 1
pubmed:dateCreated
1982-4-20
pubmed:abstractText
The ordered structure of the leading edge (lamellipodium) of cultured fibroblasts is readily revealed in cells extracted briefly in Triton X-100-glutaraldehyde mixtures, fixed further in glutaraldehyde, and then negatively stained for electron microscopy. By this procedure, the leading edge regions show a highly organised, three-dimensional network of actin filaments together with variable numbers of radiating actin filament bundles or microspikes. The use of Phalloidin after glutaraldehyde fixation resulted in a marginal improvement in filament order. Processing of the cytoskeletons though the additional steps generally employed for conventional electron microscopy resulted in a marked deterioration or complete disruption of the order of the actin filament networks. In contrast, the actin filaments of the stress fiber bundles were essentially unaffected. Thus, postfixation in osmium tetroxide (1% for 7 min at room temperature) transformed the networks to a reticulum of kinked fibers, resembling those produced by the exposure of muscle F-actin to OsO4 in vitro (P. Maupin-Szamier and T. D. Pollard. 1978. J. Cell Biol. 77:837--852). While limited exposure to OsO4 (0.2+ for 20 min at 0 degrees C) obviated this destruction, dehydration in acetone or ethanol, with or without post-osmication, caused a further and unavoidable disordering and aggregation of the meshwork filaments. The meshwork regions of the leading edge then showed a striking resemblance to the networks hitherto described in critical point-dried preparations of cultured cells. I conclude that much of the "microtrabecular lattice" described by Wolosewick and Porter (1979. J. Cell Biol. 82:114--139) in the latter preparations constitutes actin meshworks and actin filament arrays, with their associated components, that have been distorted and aggregated by the preparative procedures employed.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-1034175, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-1089542, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-1117029, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-14065312, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-156305, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-17621694, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-18683, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-28332, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-291981, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-378407, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-389938, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-407711, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-4114444, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-4181952, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-4210210, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-47197, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-479294, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-4866690, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-4873173, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-4907703, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-4933470, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-558198, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-565473, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-567223, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-569662, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-5768273, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-5884645, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-6892617, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-6893451, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-6894451, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-712845, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-728995, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-800620, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-987247, http://linkedlifedata.com/resource/pubmed/commentcorrection/6799521-988741
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
695-705
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Organization of actin in the leading edge of cultured cells: influence of osmium tetroxide and dehydration on the ultrastructure of actin meshworks.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't