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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1982-2-12
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pubmed:abstractText |
A new form of skeletal-muscle C-protein has been isolated from rabbit soleus (red) muscle. This new form of C-protein has been purified to homogeneity by a procedure similar to that used to purify C-protein from white skeletal muscle. In soleus muscle, only this new form of C-protein could be detected, whereas in psoas (white) muscle, only the previously identified form of C-protein was detected. The content of C-protein in rabbit soleus muscle is comparable with that found in psoas muscle. Other rabbit skeletal muscles composed of a mixture of fibre types contained at least two forms of C-protein. C-Protein derived from red skeletal muscle bound to myosin isolated from either red or white tissue, with maximum binding occurring at a ratio of approximately 13 microgram of red C-protein/100 microgram of myosin. Polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate indicated that C-protein isolated from red skeletal muscle has a molecular weight approx. 7% greater than that of C-protein isolated from white skeletal muscle. The amino acid content of both forms of C-protein was similar but major differences in the mol % of isoleucine and threonine were found. Antiserum against C-protein from white rabbit skeletal muscle formed a single precipitin line with rabbit C-protein on double in agar. This antiserum did not form a precipitin line when diffused against red C-protein from rabbit skeletal muscle. Also, this antiserum bound specifically to the A-band region of myofibrils isolated from psoas (white) muscle, but it did not bind to myofibrils prepared from soleus (red) muscle.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-1080204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-1100851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-122817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-12325381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-152359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-263692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-352343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-370113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-4261555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-4265361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-4269687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-4453001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-4760132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-4802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-57622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-5806584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-629749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-814246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-851428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6797400-90047
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
195
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
463-9
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:6797400-Amino Acids,
pubmed-meshheading:6797400-Animals,
pubmed-meshheading:6797400-Carrier Proteins,
pubmed-meshheading:6797400-Chickens,
pubmed-meshheading:6797400-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6797400-Fluorescent Antibody Technique,
pubmed-meshheading:6797400-Immunodiffusion,
pubmed-meshheading:6797400-Muscle Proteins,
pubmed-meshheading:6797400-Muscles,
pubmed-meshheading:6797400-Myosins,
pubmed-meshheading:6797400-Protein Binding,
pubmed-meshheading:6797400-Rabbits
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pubmed:year |
1981
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pubmed:articleTitle |
C-protein from rabbit soleus (red) muscle.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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