Linked Life Data

6796129

Source:http://linkedlifedata.com/resource/pubmed/id/6796129

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-2-22
pubmed:abstractText
The interaction between purified human factor VIII and phospholipid vesicles was investigated. The binding of factor VIII to an equimolecular mixture of phosphatidylserine (PS) and phosphatidylcholine (PC) was studied by sucrose gradient ultracentrifugation (10-40% w/v saccharose in 0.01 M Tris-HC1/0.15 M NaCl buffer (pH 7). In the absence of phospholipids all factor VIII activities (VIII R : WF and VIII R : AG) were found in the zone of highest sucrose density including the factor VIII related protein subunit (200 000 molecular weight). In the presence of an equimolecular mixture of PS/PC VIII R : WF activity, VIII R: AG and factor VIII related protein still migrated to the bottom of the tube, while VIII: C activity remained at the top where phospholipids were found. Thus a dissociation phenomenon between VIII : C and the other factor VIII related activities was apparent in the presence of phospholipids. These results also demonstrate the binding of factor VIII: C to certain active phospholipids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
678
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
132-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Human factor VIII procoagulant activity and phospholipid interaction.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't