Linked Life Data

6795704

Source:http://linkedlifedata.com/resource/pubmed/id/6795704

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1982-1-9
pubmed:abstractText
Subfractions of human glomerular basement membrane isolated by enzymatic procedures were analyzed for their chemical structure and biological activity. Pepsin or collagenase digestion of purified basement membranes released three groups of components: collagenous and noncollagenous fractions and also a mixed material consisting of a proteolysis-resistant association between collagen sequences and heteropolysaccharidic glycopeptides. These different fractions were explored in vitro for their ability to interact with cell membranes. In fact, only fractions containing the heterogenous material agglutinated human transformed or embryonic cells within 2 h. The cell agglutination was specifically inhibited by N-acetylosamines and N-acetylneuraminic acid. An additional incubation of 20 h at 37 degrees C of the agglutinated cells, maintained in a minimal medium devoid of serum, led to a morphological change of the transformed cells due to an increased cell adhesion and cell spreading. These experimental data suggest that the basement membrane possesses active sites - consisting of an association between collagen and structural glycoproteins - which could play a primordial role in cell matrix interactions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0378-5858
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
67-73
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Chemical characterization and functional role of human glomerular basement membrane components.
pubmed:publicationType
Journal Article