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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1981-12-22
|
| pubmed:abstractText |
As a rule, Proteus morganii does not decarboxylate lysine. However, lysine-positive P. morganii strains have been recently described. We suspected a plasmid origin for this atypical character, and we analyzed 14 strains to study this question. Among these strains, 8 yielded lysine-negative segregants after acridine orange or ethidium bromide treatment, and 10 transferred their lysine-positive character to a recipient P. morganii strain. All of the 14 strains analyzed at least segregated or conjugated. Three lysine-positive transconjugants, in turn, segregated lysine-negative variants after ethidium bromide treatment. The eight wild-type lysine-positive strains that segregated lysine-negative subclones contained a large (35 to 45 megadaltons) plasmid detectable by agarose gel electrophoresis. Similarly, the 10 lysine-positive transconjugants contained a plasmid of the same size, whereas P. morganii 1000, the recipient strain, did not contain any detectable plasmid. The large plasmid clearly disappeared in 9 of 11 lysine-negative segregants analyzed. It is concluded that the lysine-positive character of these P. morganii strains is plasmid encoded, and the taxonomical implications are discussed.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-1092355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-1092649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-13920003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-16561959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-349354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-391817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-4564488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-4600107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-4934056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-5325707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-6248727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-6775010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-6989959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-814405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6793621-956120
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0095-1137
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
365-9
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading | |
| pubmed:year |
1981
|
| pubmed:articleTitle |
Plasmid-encoded lysine decarboxylation in Proteus morganii.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|