Linked Life Data

6791511

Source:http://linkedlifedata.com/resource/pubmed/id/6791511

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-10-14
pubmed:abstractText
We analyzed brain tissue for adenosine and its metabolites, inosine and hypoxanthine, in rats during acute (30 s) hypoxia and during sustained (5 min) hypoxia and hypocarbia. Within 30 s of the onset of hypoxia, adenosine levels were increased from 0.34 +/- 0.08 (SE) to 1.65 +/- 0.33 nmol/g (P less than 0.005), paralleling temporally the changes in cerebral blood flow. During sustained hypoxia and hypocarbia, brain tissue was sampled by a fast (freeze-blow) or slow (in situ) freezing method. With the freeze-blow technique, adenosine concentrations remained stable between arterial partial pressure of O2 (PaO2) greater than 200 and 100 mmHg, doubled at PaO2 = 50 mmHg, and increased sevenfold (P less than 0.005) when PaO2 reached 30 mmHg. No increases in adenosine or its metabolites were noted with the in situ technique. During hypocarbia (arterial CO2 partial pressure less than 20 mmHg), adenosine concentrations increased with both sampling techniques. Freezing times in brain were measured during in situ freezing and were increased during hypoxia and decreased during hypocarbia. In conclusion, 1) adenosine concentrations in brain are increased during hypoxia, and 2) the in situ technique in rat does not appear to be optimal for sampling brain tissue for subsequent measurement of adenosine under conditions where cerebral blood flow is increased.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
241
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
H235-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Brain adenosine concentration during hypoxia in rats.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't