Linked Life Data

6790571

Source:http://linkedlifedata.com/resource/pubmed/id/6790571

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-10-14
pubmed:abstractText
The Phadebact Gonococcus Test (Pharmacia Diagnostics, Piscataway, N.J.), a coagglutination technique, was compared with the rapid fermentation method of Kellogg and Turner (D. S. Kellogg, Jr., and E. M. Turner, Appl. Microbiol. 25: 550--552, 1973). A total of 93 organisms isolated on Martin-Lewis media were determined to be Neisseria gonorrhoeae based on the following criteria: presence of gram-negative diplococci, oxidase positivity, and appropriate reaction in the rapid fermentation method. These 93 isolates were then serologically tested with the Phadebact test. The direct method was attempted on the first 46 N. gonorrhoeae isolates. Difficulty in interpreting results was encountered in 39%. Thereafter, the alternate method of boiling was instituted on an additional 47 N. gonorrhoeae isolates, with 2 isolates producing noninterpretable results. All 93 isolates were frozen for a maximum of 2 months in skim milk at -25 degrees C. These isolates were thawed and retyped with the alternate boiling procedure, with 97% being confirmed as N. gonorrhoeae. In addition, 33 Neisseria meningitidis isolates, 14 Neisseria species, and 7 Moraxella species were tested with similar techniques. No positive reactions were observed. A cost effectiveness study using 5, 10, and 20 microliters of the gonococcal reagent was undertaken to reduce the cost of the test. When 10 and 20 microliters of reagent were used, no difficulty was encountered in interpreting the reaction. The coagglutination technique was difficult to read when 5 microliters of reagent was used.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-114531, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-14061556, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-4145340, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-4207280, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-4598431, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-4633478, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-6766952, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-6768769, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-6773645, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-6775005, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-6776142, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-809474, http://linkedlifedata.com/resource/pubmed/commentcorrection/6790571-829828
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
89-93
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Comparison of the Phadebact Gonococcus Test with the rapid fermentation method.
pubmed:publicationType
Journal Article, Comparative Study