Linked Life Data

6787134

Source:http://linkedlifedata.com/resource/pubmed/id/6787134

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-8-10
pubmed:abstractText
Conditions are defined for the in vitro growth of antibody producing hybrid myeloma cell lines in an immediate environment free of non-dialyzable serum components. Continuous access to low molecular weight serum growth factors was critical to the maintenance of cell viability and antibody production. This requirement was met by use of two-chambered Marbrook vessels, whose geometry allows a small chamber containing metabolically active cells to communicate through a dialysis membrane with a large adjacent reservoir filled with serum-supplemented medium. This tissue culture technique permits the rapid isolation of proteins secreted by cells in vitro, and should permit the further definition of minimal nutritional requirements for the growth of differentiated cells in vitro.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Dialyzable serum components can support the growth of hybridoma cell lines in vitro.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.