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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1978-9-15
|
| pubmed:abstractText |
A micromethod is described that uses whole blood for studying mitogen stimulation of chicken lymphocytes. Procedures critical for obtaining a stimulatory response up to 200 times that of unstimulated control cultures include: 1) use of no more than 10 microliter of whole blood per culture; 2) incubation at 41 C for 72 hours in 0.2 ml of medium RPMI 1640 containing 100 microgram of stock phytohemagglutinin-P and 3) pulse labeling for 8 hours with 5-[125I] iodo-2'-deoxyruidine. The method is easy, rapid, and suitable for sequential studies of pathogenesis of disease in poultry.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0005-2086
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
296-307
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:678234-Animals,
pubmed-meshheading:678234-Blood,
pubmed-meshheading:678234-Chickens,
pubmed-meshheading:678234-Concanavalin A,
pubmed-meshheading:678234-Culture Media,
pubmed-meshheading:678234-Lectins,
pubmed-meshheading:678234-Lymphocyte Activation,
pubmed-meshheading:678234-Spleen,
pubmed-meshheading:678234-Temperature
|
| pubmed:articleTitle |
Chicken lymphocyte stimulation by mitogens: a microassay with whole-blood cultures.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|