Linked Life Data

6782187

Source:http://linkedlifedata.com/resource/pubmed/id/6782187

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1981-5-21
pubmed:abstractText
An electroimmunodiffusion assay (EID) for the quantitative determination of the major apolipoproteins of the human high density lipoprotein fraction, apolipoproteins A-I and A-II, was compared with a solid phase radioimmunoassay (RIA), previously described by us. Data obtained by both methods largely depended on the pretreatment of the sera. Pretreatment consisted either of delipidation with an ether/ethanol mixture or tetramethyl urea, or of denaturation by heating the sera to 52 degree C for 3 hours. Delipidation with ether/ethanol produced a decrease of apolipoprotein A-I values in both methods as compared to treatment with tetramethyl urea. In contrast, the ether/ethanol treatment did not alter apolipoprotein A-II values in the RIA, while in the EID this procedure led to an increase of apolipoprotein A-II values. Heating gave an increase of both apolipoproteins A-I and A-II in both assays, but results obtained by EID were difficult to interpret because of the appearance of indistinct or double rockets. Comparable results by both methods were obtained, when sera were pretreated with tetramethyl urea. Handling and reproducibility seemed to be slightly better with the EID than with the RIA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0340-076X
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
917-20
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Electroimmunoassay and radioimmunoassay for the quantitation of high density apolipoproteins A-I and A-II.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't