Linked Life Data

6781757

Source:http://linkedlifedata.com/resource/pubmed/id/6781757

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-5-28
pubmed:abstractText
During B cell differentiation to pentamer IgM secretion, synthesis of the pentamer joining component, the J chain, is initiated. We investigated the mechanism for initiating J chain synthesis by analyzing murine cell lines representing different stages in B cell differentiation. The expression of functional J chain mRNA was evaluated by cell-free translation and specific immunoprecipitation of a J chain product. The expression of precursor mRNA was examined by hybridization with a J chain probe obtained by molecular cloning of cDNA. No J chain-specific RNA could be demonstrated in a lymphoma line representative of an undifferentiated B lymphocyte, but three species of J chain RNA were identified in hybrid cell lines representative of IgM-secreting plasma cells: a mature message of approximately 1.5 kb and two minor components of 2.5 and 0.92 kb. The encounter of a B cell with antigen or mitogen must therefore trigger events that effect either transcription of J chain sequences or their intranuclear stabilization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
369-78
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Expression of J chain RNA in cell lines representing different stages of B lymphocyte differentiation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't