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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1981-5-28
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pubmed:abstractText |
During B cell differentiation to pentamer IgM secretion, synthesis of the pentamer joining component, the J chain, is initiated. We investigated the mechanism for initiating J chain synthesis by analyzing murine cell lines representing different stages in B cell differentiation. The expression of functional J chain mRNA was evaluated by cell-free translation and specific immunoprecipitation of a J chain product. The expression of precursor mRNA was examined by hybridization with a J chain probe obtained by molecular cloning of cDNA. No J chain-specific RNA could be demonstrated in a lymphoma line representative of an undifferentiated B lymphocyte, but three species of J chain RNA were identified in hybrid cell lines representative of IgM-secreting plasma cells: a mature message of approximately 1.5 kb and two minor components of 2.5 and 0.92 kb. The encounter of a B cell with antigen or mitogen must therefore trigger events that effect either transcription of J chain sequences or their intranuclear stabilization.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0092-8674
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
369-78
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6781757-Animals,
pubmed-meshheading:6781757-B-Lymphocytes,
pubmed-meshheading:6781757-Cell Differentiation,
pubmed-meshheading:6781757-Cell Line,
pubmed-meshheading:6781757-Cloning, Molecular,
pubmed-meshheading:6781757-Immunoglobulin J-Chains,
pubmed-meshheading:6781757-Immunoglobulin M,
pubmed-meshheading:6781757-Mice,
pubmed-meshheading:6781757-Nucleic Acid Hybridization,
pubmed-meshheading:6781757-Plasmacytoma,
pubmed-meshheading:6781757-RNA, Messenger
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pubmed:year |
1981
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pubmed:articleTitle |
Expression of J chain RNA in cell lines representing different stages of B lymphocyte differentiation.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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