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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1980-11-25
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pubmed:abstractText |
A specialized Mu transducing phage containing a gene encoding ampicillin resistance and the lac structural genes without the lac promotor [Mu d(apr lac)] has been constructed and used to create gene fusions in Escherichia coli (M. J. Cadadaban and S. N. Cohen, Proc. Natl. Acad. Sci. U.S.A. 76:4530--4533, 1979). Transposition of the Mu d(Apr lac) phage to chromosomal sites can result in lac expression being controlled by a chromosomal promoter. We have constructed an Escherichia coli K-12 strain in which the Mu d(Apr lac) phage is integrated into an F factor. The F+::Mu d(Apr lac) was then transferred by conjugation into a Salmonella typhimurium strain that was sensitive to L-arabinose. Strains containing gene fusions were selected as L-arabinose-resistant colonies after partial induction of the phage. Two classes of ara-lac fusion strains were isolated: (i) araC-lac fusions in which the expression of beta-galactosidase synthesis was constitutuve and not inducible by L-arabinose; and ((ii) fusion of the lac genes to the ara structural genes in which the expression of beta-galatosidase synthesis was induced 263-fold by L-arabinose.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-1093171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-113385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-13696894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-13872181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-14096176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-14168696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-159458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-17248903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-344891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-353483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-4323960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-4560760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-4568766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-4932889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-5330661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-6021093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-6997720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6773928-781294
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1325-31
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:6773928-Arabinose,
pubmed-meshheading:6773928-Coliphages,
pubmed-meshheading:6773928-Galactosidases,
pubmed-meshheading:6773928-Gene Expression Regulation,
pubmed-meshheading:6773928-Genes,
pubmed-meshheading:6773928-Operon,
pubmed-meshheading:6773928-Recombination, Genetic,
pubmed-meshheading:6773928-Salmonella typhimurium,
pubmed-meshheading:6773928-Transduction, Genetic,
pubmed-meshheading:6773928-beta-Galactosidase
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pubmed:year |
1980
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pubmed:articleTitle |
Isolation of ara-lac gene fusions in Salmonella typhimurium LT2 by using transducing bacteriophage Mu d (Apr lac).
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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