Linked Life Data

6769482

Source:http://linkedlifedata.com/resource/pubmed/id/6769482

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1980-8-28
pubmed:abstractText
1. The outer membrane of a phospholipase A-deficient mutant of Escherichia coli K12, isolated without the use of EDTA and lysozyme, showed the same freeze-fracture morphology as that seen in cells and remained stable for hours as observed by 31P-NMR. 2. 31P-NMR spectroscopy of the isolated outer membranes revealed that the lipopolysaccharide exists in the same physical state as in phospholipid-lipopolysaccharide liposomes and is most probably arranged in a bilayer at 37 degrees C. The outer membrane contains most or all of the phospholipids at 37 degrees C, and all the phospholipids at 20 degrees C, as a bilayer. 3. The 31P-NMR spectroscopy of the outer membranes from a mutant strain lacking the major outer membrane protein b, c and d (60% of the total outer membrane protein) yields virtually the same spectrum as the wild-type outer membranes, although most of the particles and pits which were observed in wild-type outer membranes in freeze-fracture electron microscopy were absent. 4. Whereas treatment of wild-type outer membranes with calcium ions has no effect on the 31P-NMR spectrum, treatment with EDTA results in more motion of the lipopolysaccharide.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
597
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
518-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
31P nuclear magnetic resonance and freeze-fracture electron microscopy studies on Escherichia coli. III. The outer membrane.
pubmed:publicationType
Journal Article, Comparative Study