Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1980-7-12
pubmed:abstractText
Semliki Forest virus (SFV)-infected BHK-21, Vero, and HeLa cells incorporated [3H]uridine into 42S and 26S plus-strand RNA and into viral minus-strand RNA (complementary to the 42S virion RNA) early in the infectious cycle. Between 3 and 4 h postinfection, the synthesis of minus-strand RNA ceased in these cultures, although the synthesis of plus-strand RNA continued at a maximal rate. At the time of cessation of minus-strand RNA synthesis, two changes in the pattern of viral protein synthesis were detected: a decrease in the translation of nonstructural proteins and an increase in the translation of the viral structural proteins. Addition of cycloheximide and puromycin to cultures of SFV-infected BHK cells actively synthesizing both viral plus- and minus-strand RNA resulted within 15 to 30 min in the selective shutoff of minus-strand RNA synthesis. Removal of the cycloheximide-containing medium led to the resumption of minus-strand synthesis and to an increased rate of viral RNA synthesis. We conclude that the minus-strand polymerase regulates the rate of SFV plus-strand RNA synthesis by determining the number of minus-strand templates and that the synthesis of the minus-strand templates is regulated at the level of translation by a mechanism which utilizes one or more short-lived polymerase proteins.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-1159408, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-1169852, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-165500, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-16789106, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-223155, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-365471, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-4342056, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-4355930, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-4357512, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-4365420, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-4398631, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-4675143, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-4833537, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-4843746, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-506064, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-5165207, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-5229967, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-5798245, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-5817400, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-5817532, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-621775, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-965947, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-965948, http://linkedlifedata.com/resource/pubmed/commentcorrection/6768898-978799
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
108-18
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Short-lived minus-strand polymerase for Semliki Forest virus.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.