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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1980-5-14
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pubmed:abstractText |
The role of Ca2+ in toxic liver cell death was studied with primary cultures of adult rat hepatocytes. Within 1 hr of exposure to phalloidin, a bicyclic heptapeptide isolated from the mushroom Amanita pahlloides, at 50 micrograms/ml, 60--70% of the cells were dead (trypan blue stainable). There was no loss of viability of the same cells exposed to phalloidin in culture medium devoid of Ca2+. A marked structural alteration of the surface of the phalloidin-treated hepatocytes characterized by innumerable evaginations seen by scanning electron microscopy occurred in the presence or absence of Ca2+. Pretreatment of the cells with cytochalasin B at 10 micrograms/ml prevented the surface alteration and the death of the cells in Ca2+ medium. Exposure of the cells to phalloidin in the absence of Ca2+ followed by exposure to cytochalasin B and then to Ca2+ also prevented the cell death. These results suggest a two-step mechanism by which phalloidin causes liver cell death. Initially phalloidin interacts in a Ca2+-independent process with cell membrane-associated actin. The second step is a Ca2+-dependent process that most likely represents an increased influx of Ca2+ across a compromised cell membrane permeability barrier and down the steep concentration gradient that exists between the outside and inside of the cell. These results strengthen the hypothesis that disturbances in Ca2+ homeostasis induced in vivo by a variety of hepatotoxins are causally related to liver cell death.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-1137005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-124264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-1249072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-126084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-132190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-13367995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-13740295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-13986421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-14069791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-14212869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-14226507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-33670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-363352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-386513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-4213453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-4254274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-4258316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-4262099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-4368830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-4719528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-5124481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-5326167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-5931974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-786156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-844802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-859648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6767245-987016
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1177-80
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6767245-Actins,
pubmed-meshheading:6767245-Animals,
pubmed-meshheading:6767245-Calcimycin,
pubmed-meshheading:6767245-Calcium,
pubmed-meshheading:6767245-Cell Survival,
pubmed-meshheading:6767245-Cells, Cultured,
pubmed-meshheading:6767245-Cytochalasin B,
pubmed-meshheading:6767245-Cytoskeleton,
pubmed-meshheading:6767245-Drug Synergism,
pubmed-meshheading:6767245-Female,
pubmed-meshheading:6767245-Liver,
pubmed-meshheading:6767245-Oligopeptides,
pubmed-meshheading:6767245-Phalloidine,
pubmed-meshheading:6767245-Rats
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pubmed:year |
1980
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pubmed:articleTitle |
Calcium dependence of phalloidin-induced liver cell death.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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