Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1988-2-10
pubmed:databankReference
pubmed:abstractText
Plasmids containing the nontranscribed central and terminal, but not the coding, regions of the extrachromosomal ribosomal deoxyribonucleic acid (rDNA) of Tetrahymena thermophila are capable of autonomous replication in Saccharomyces cerevisiae. These plasmids transform S. cerevisiae at high frequency; transformants are unstable in the absence of selection, and plasmids identical to those used for transformation were isolated from the transformed yeast cells. One plasmid contains a 1.85-kilobase Tetrahymena DNA fragment which includes the origin of bidirectional replication of the extrachromosomal rDNA. The other region of Tetrahymena rDNA allowing autonomous replication of plasmids in S. cerevisiae is a 650-base pair, adenine plus thymine-rich segment from the rDNA terminus. Neither of these Tetrahymena fragments shares obvious sequence homology with the origin of replication of the S. cerevisiae 2-microns circle plasmid or with ars1, an S. cerevisiae chromosomal replicator.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-1033038, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-1105072, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-1105573, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-149110, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-242001, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-265521, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-347451, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-357984, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-375221, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-387728, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-387734, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-388229, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-388424, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-406929, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-407400, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-409501, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-413699, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-4372397, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-4469597, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-4528573, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-455443, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-4559594, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-4600264, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-488177, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-5231757, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-6248420, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-6251374, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-6256739, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-6257908, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-6266918, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-6271358, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-642006, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-6449009, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-6987527, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-6987539, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-786462, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-820863, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-820864, http://linkedlifedata.com/resource/pubmed/commentcorrection/6765606-881736
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
535-43
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae.
pubmed:affiliation
Department of Biology, York University, Downsview, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't