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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1983-5-5
|
| pubmed:abstractText |
Factors influencing the preservation of HBcAg in formalin-fixed, paraffin-embedded liver tissue have been analysed. Fixation time, choice of the embedding medium, deparaffinization and selection of anti-HBc proved to be critical variables. For optimal results, fixation in 4% buffered formalin for no longer than 24-48 h, the use of wax with a low melting point, a heating step prior to deparaffinization, and a search for anti-HBc specifically reactive for paraffinated HBcAg are recommended.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0106-9543
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
331-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6762478-Fixatives,
pubmed-meshheading:6762478-Fluorescent Antibody Technique,
pubmed-meshheading:6762478-Formaldehyde,
pubmed-meshheading:6762478-Frozen Sections,
pubmed-meshheading:6762478-Hepatitis B Core Antigens,
pubmed-meshheading:6762478-Histological Techniques,
pubmed-meshheading:6762478-Humans,
pubmed-meshheading:6762478-Liver,
pubmed-meshheading:6762478-Paraffin
|
| pubmed:year |
1982
|
| pubmed:articleTitle |
An improved method for HBcAg demonstration in paraffin-embedded liver tissue.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|