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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1983-3-11
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pubmed:abstractText |
Resident mouse peritoneal macrophages release the slow-reacting substance leukotriene C (LTC) on exposure to particulate IgE immune complexes. Because these cells lose their responsiveness to an IgE stimulus after 4 h in culture, maximum release of 20:4 metabolites is observed before this time. However, a similar diminution in 20:4 metabolism was not observed with a zymosan stimulus. Freshly explanted cells are deficient in intracellular glutathione (GSH) (12.4 +/- 0.4 pmol/micrograms cell protein), but GSH increases to a steady state value of 30-35 pmol/micrograms of cell protein between 3 and 9 h of culture. Because GSH is required for the synthesis of LTC and prostaglandin (PG)E2, cultures challenged immediately after explanation have a diminished capacity to synthesize these 20:4 metabolites and release prostacyclin as the major product. By 4-5 h in culture, macrophages form significant amounts of LTC and PGE2. Under optimum conditions of maximum responsiveness to an IgE stimulus and GSH content (after 4 h of culture), macrophages challenged with latex beads coated with IgE immune complexes synthesize 1.0 +/- 0.3 pmol of LTC/microgram cell protein (60 +/- 18 pmol/10(6) cells) in addition to prostacyclin (8.2 +/- 0.8 pmol/micrograms cell protein) and PGE2 (4.7 +/- 1.5 pmol/micrograms cell protein). These amounts are quantitatively similar to the arachidonic acid metabolites produced by macrophages challenged with IgG immune complex-coated latex beads or zymosan. These data demonstrate that macrophages produce large quantities of LTC and other 20:4 metabolites in response to particle-bound IgE and antigen, provided that the appropriate in vitro conditions are met. The macrophage might, therefore, be a major source of slow-reacting substance and other 20:4 metabolites generated during IgE-mediated reactions in vivo.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-111577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-13809017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-323399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-41240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-43155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-4940429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-6107327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-6120172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-6120989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-6256467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-6803245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-6933478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-7373045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-7400759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-7416462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6759607-743251
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
156
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1077-86
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6759607-Animals,
pubmed-meshheading:6759607-Antigen-Antibody Complex,
pubmed-meshheading:6759607-Cells, Cultured,
pubmed-meshheading:6759607-Epoprostenol,
pubmed-meshheading:6759607-Female,
pubmed-meshheading:6759607-Immunoglobulin E,
pubmed-meshheading:6759607-Macrophages,
pubmed-meshheading:6759607-Mice,
pubmed-meshheading:6759607-Mice, Inbred ICR,
pubmed-meshheading:6759607-Prostaglandins,
pubmed-meshheading:6759607-Prostaglandins E,
pubmed-meshheading:6759607-SRS-A,
pubmed-meshheading:6759607-Time Factors
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pubmed:year |
1982
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pubmed:articleTitle |
Secretion of leukotriene C and other arachidonic acid metabolites by macrophages challenged with immunoglobulin E immune complexes.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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