Linked Life Data

6758425

Source:http://linkedlifedata.com/resource/pubmed/id/6758425

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-2-14
pubmed:abstractText
Different chromatographic methods are described to remove traces of LPS from proteins extracted from the surface of several R-mutants of Salmonella. The protein preparations obtained by these methods were found to contain less than 0.001% of beta-hydroxymyristic acid as determined by gas liquid chromatography. This corresponds to less than 0.003 to 0.006% of R-form-LPS (depending on the chemotype of the mutant) and less than 0.014% of S-form-LPS. From some protein mixtures LPS could be removed by repeated gel filtration on a column of Sepharose CL-6B. Recycling gel filtration proved to be more effective considering the high recovery of protein in much shorter time. Ion exchange chromatography on DEAE Sepharose CL-6B was also useful in some cases. Thus, using one or the other method LPS free protein preparations could be obtained with extracts obtained from 6 out of 7 bacterial strains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0174-3031
pubmed:author
pubmed:issnType
Print
pubmed:volume
253
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
88-101
pubmed:dateRevised
2009-6-8
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Proteins from Salmonella R-mutants mediating protection against Salmonella typhimurium infection in mice I. Preparation of proteins free from lipopolysaccharide using various chromatographic methods.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't