Linked Life Data

6757924

Source:http://linkedlifedata.com/resource/pubmed/id/6757924

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1983-2-25
pubmed:abstractText
To develop techniques for studying transport properties and secretory function of selected cell types in the gastric mucosa, separated fractions of dispersed canine fundic mucosal cells were placed in short-term culture to form epithelial monolayers. Cell fractions enriched in either chief, parietal, or mucous cells were prepared by using counterflow centrifugation and were plated on type I collagen. An epithelial monolayer formed by approximately equal to 36 hr. Immunofluorescence with an antipepsinogen I antibody revealed pepsinogen-containing granules in greater than 95% of the cells, regardless of whether the monolayers were formed from the mucous, chief, or parietal cell-enriched fractions. Upon achieving confluency, chief cell monolayers were mounted in Ussing chambers to study their electrical properties. Under basal conditions, monolayers (n = 6) had a spontaneous potential difference (PD) (+/- SEM) of 26 +/- 4 mV (apical surface negative), a short-circuit current (Isc) (+/- SEM) of 16 +/- 2 microA/cm2, and a transepithelial resistance (R) (+/- SEM) of 1,480 +/- 210 omega X cm2. Histamine increased the short-circuit current, an effect blocked by an H2-receptor antagonist. Seventy percent of the spontaneous PD was amiloride sensitive, suggesting sodium absorption accounted for a major component of the PD. These preparative techniques yield highly enriched chief cell monolayers, which maintain morphological and functional cellular differentiation for greater than 48 hr in culture, thus allowing study of oriented functions of a selected cell type. The present studies indicate that an H2 receptor enhances electrogenic ion transport in chief cell monolayers, indicating that histamine can act on fundic mucosal cells other than just parietal cells.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-1063404, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-227276, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-284373, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-4124404, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-4917140, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-4976023, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-559643, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-567227, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-6104278, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-621277, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-7129478, http://linkedlifedata.com/resource/pubmed/commentcorrection/6757924-7241572
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7009-13
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Electrical effects of histamine on monolayers formed in culture from enriched canine gastric chief cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.