Linked Life Data

6752573

Source:http://linkedlifedata.com/resource/pubmed/id/6752573

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1982-12-21
pubmed:abstractText
The geometry of the vascular system is such that the surface area to volume ratio changes dramatically as a unit volume of blood circulates. Thus, it faces a surface area that is some 1000 times larger in the capillaries than in larger vessels. To approach the in vivo situation an in vitro model for the microcirculation was developed that uses endothelium cultured on microcarrier beads. Details of the culture method are presented. The cultures form confluent monolayers around the beads and resist removal by vigorous vortex mixing. Viability of the cells on the microcarrier is 100 per cent. The microcarrier cultures are packed into small columns (0.3 to 1.0 ml.) forming a perfusion system and used for studies of the binding of thrombin and platelet factor 4, for the demonstration of anticoagulant properties of the cells, for studies of the interaction between endothelium and platelets, and for the demonstration of prostacyclin release.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0023-6837
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
498-504
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Use of endothelium cultured on microcarriers as a model for the microcirculation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't