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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1982-12-3
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pubmed:abstractText |
A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1-transformed NP37 overproduce initiation factor 3 and phenylalanyl-tRNA synthetase. Gene expression from pID1 was studied in vitro in a coupled transcription-translation system and in minicells. The results suggest that the genes for initiation factor 3 and phenylalanyl- and threonyl-tRNA synthetase are regulated by different mechanisms.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-105669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-1108000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-1180514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-14273649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-149110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-16591472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-318645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-327276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-4559594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-4598754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-4935321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-543547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-6166752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-6254853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-6264314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-6445562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-7009546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-7012833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6749810-7031052
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
152
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
357-62
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:6749810-Amino Acyl-tRNA Synthetases,
pubmed-meshheading:6749810-Cloning, Molecular,
pubmed-meshheading:6749810-Escherichia coli,
pubmed-meshheading:6749810-Gene Expression Regulation,
pubmed-meshheading:6749810-Genes, Bacterial,
pubmed-meshheading:6749810-Peptide Initiation Factors,
pubmed-meshheading:6749810-Phenylalanine-tRNA Ligase,
pubmed-meshheading:6749810-Plasmids,
pubmed-meshheading:6749810-Prokaryotic Initiation Factor-3,
pubmed-meshheading:6749810-Threonine-tRNA Ligase
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pubmed:year |
1982
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pubmed:articleTitle |
Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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