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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0001675,
umls-concept:C0014442,
umls-concept:C0018787,
umls-concept:C0021289,
umls-concept:C0030685,
umls-concept:C0242184,
umls-concept:C0243144,
umls-concept:C0391871,
umls-concept:C0439849,
umls-concept:C0596235,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1522564,
umls-concept:C1963578
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pubmed:issue |
6
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pubmed:dateCreated |
1984-9-7
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pubmed:abstractText |
The relationship between creatine phosphokinase (CPK) release and sarcolemmal permeability to divalent cations (Ca2+ and Ba2+) during hypoxia and reoxygenation was studied in the isolated arterially perfused septal preparation of the newborn and adult rabbit. Tissue 47Ca2+ or 133Ba2+ uptake was measured by a juxtaposed gamma-probe. Since Ba2+ is not taken up by the sarcoplasmic reticulum and mitochondria, 133Ba2+ was used to determine sarcolemmal permeability to divalent cations (Ca2+ and Ba2+). In the two age groups, tissue Ca2+ uptake was unchanged during hypoxia and increased significantly during reoxygenation. Ba2+ uptake remained unchanged during hypoxia and reoxygenation. CPK release was small during hypoxia and increased significantly during reoxygenation. The increases in tissue Ca2+ uptake and CPK release in the newborn were significantly less than in the adult. Perfusion with low Ca2+ solutions (0.3 mM, 0.5 mM and 'zero') decreased tissue Ca2+ gain but did not prevent CPK release during reoxygenation. In the muscle perfused with an oxygenated solution containing phospholipase C (0.1 U/ml), the rate of CPK release increased significantly, but tissue Ca2+ uptake and Ba2+ uptake remained unchanged. These data suggest that: (1) sarcolemmal damage (evidenced by enzyme release) during hypoxia and reoxygenation in the newborn is less than in the adult. (2) enzyme release and tissue Ca2+ gain can occur during reoxygenation without significant changes in sarcolemmal permeability to divalent cations (Ca2+ and Ba2+) that can be detected by the present techniques, and (3) enzyme release during reoxygenation is associated with but may not be caused by the increased tissue Ca2+.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Barium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Creatine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-2828
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
519-32
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6748087-Animals,
pubmed-meshheading:6748087-Animals, Newborn,
pubmed-meshheading:6748087-Anoxia,
pubmed-meshheading:6748087-Barium,
pubmed-meshheading:6748087-Calcium,
pubmed-meshheading:6748087-Creatine Kinase,
pubmed-meshheading:6748087-Myocardium,
pubmed-meshheading:6748087-Oxygen,
pubmed-meshheading:6748087-Perfusion,
pubmed-meshheading:6748087-Phosphates,
pubmed-meshheading:6748087-Phospholipases,
pubmed-meshheading:6748087-Rabbits,
pubmed-meshheading:6748087-Tissue Distribution
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pubmed:year |
1984
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pubmed:articleTitle |
The relationship between myocardial enzyme release and Ca2+ uptake during hypoxia and reoxygenation in the newborn and adult heart.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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