Linked Life Data

6734741

Source:http://linkedlifedata.com/resource/pubmed/id/6734741

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-8-1
pubmed:abstractText
The kinetics of myoblast fusion were estimated using two complementary assays. Both utilized suspensions of fusion-competent cells, i.e. 48-52-h cultures of chick pectoral muscle grown in a low-calcium medium, thus minimizing contributions arising from cellular migration. One assay, designed to measure the onset of membrane contiguity, relies on the transfer of a lipid dye, diI-C18-[3], from labelled to unlabelled cells. The other assay, designed to estimate the kinetics of appearance of morphologically distinct multinucleate cells in suspension (myoballs), relies on enzymic dissociation of cellular aggregates followed by nuclear staining. The assays demonstrate significant membrane contiguity within 20-30 min after initiating the fusion process; however, the multinucleate myoball morphology does not appear for at least one additional hour.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25-31
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
The kinetics of myoblast fusion.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't