rdf:type |
|
lifeskim:mentions |
umls-concept:C0001613,
umls-concept:C0007776,
umls-concept:C0010405,
umls-concept:C0010406,
umls-concept:C0022646,
umls-concept:C0022655,
umls-concept:C0022702,
umls-concept:C0034493,
umls-concept:C0037633,
umls-concept:C0042444,
umls-concept:C0348005,
umls-concept:C1280500,
umls-concept:C1516048,
umls-concept:C1519355,
umls-concept:C1521761
|
pubmed:issue |
3
|
pubmed:dateCreated |
1984-8-1
|
pubmed:abstractText |
A renal cortical slice model was used to assess the effects on viability of three vehicle solutions-Krebs-Henseleit (K-H), solution A, and RPS-2--at 25 degrees C. After 120 min incubation no differences in [K+]/[Na+] ratios were found. Tracer techniques were used to study the osmotic effects and permeation kinetics at 25 degrees C of three cryoprotectants (dimethyl sulfoxide (Me2SO), ethylene glycol, and glycerol) and the effect of the vehicle solution (K-H or RPS-2) on Me2SO kinetics. It was found that Me2SO was most permeable and ethylene glycol least, and that ethylene glycol had unusual effects which suggest that it may not act as a simple solute. Differences were found when Me2SO was introduced in K-H and RPS-2 that are believed to be related to the binding properties of Me2SO to cell constituents.
|
pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0011-2240
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
21
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
260-73
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:6734239-Animals,
pubmed-meshheading:6734239-Cell Membrane Permeability,
pubmed-meshheading:6734239-Cell Survival,
pubmed-meshheading:6734239-Cryoprotective Agents,
pubmed-meshheading:6734239-Dimethyl Sulfoxide,
pubmed-meshheading:6734239-Ethylene Glycol,
pubmed-meshheading:6734239-Ethylene Glycols,
pubmed-meshheading:6734239-Female,
pubmed-meshheading:6734239-Freezing,
pubmed-meshheading:6734239-Glycerol,
pubmed-meshheading:6734239-Kidney,
pubmed-meshheading:6734239-Kidney Cortex,
pubmed-meshheading:6734239-Kinetics,
pubmed-meshheading:6734239-Male,
pubmed-meshheading:6734239-Organ Preservation,
pubmed-meshheading:6734239-Osmosis,
pubmed-meshheading:6734239-Rabbits,
pubmed-meshheading:6734239-Solutions,
pubmed-meshheading:6734239-Time Factors
|
pubmed:year |
1984
|
pubmed:articleTitle |
Factors influencing renal cryopreservation. I. Effects of three vehicle solutions and the permeation kinetics of three cryoprotectants assessed with rabbit cortical slices.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|