Linked Life Data

6733129

Source:http://linkedlifedata.com/resource/pubmed/id/6733129

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-7-27
pubmed:abstractText
Fluorescence polarization measurements with 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to monitor phase changes in sonicated dispersions of triacylglycerols, cholesteryl esters and phospholipids. Lipid transitions to a fluid state were detected in a novel way by noting the temperature, t0.08, at which rs, the steady state anisotropy, was equal to 0.08. According to published equations (Van Blitterswijk , W.J., Van Hoeven , R.P. and Van der Meer , B.W. (1981) Biochim. Biophys. Acta 644, 323-332), this value for rs corresponds to a value of zero for S, a structural order parameter. Saturated and trans monounsaturated fatty triacylglycerols and distearoylphosphatidylcholine yielded t0.08 values in close agreement with transition temperatures found by differential scanning calorimetry (DSC), whereas cis unsaturated triacylglycerols displayed residual anisotropy, rs greater than 0.08, at temperatures above the DSC transition. The bent configuration of the cis double-bonded fatty acyl chains probably limits freedom of movement even in the liquid state when three such chains are bound to the glycerol molecule. Cholesteryl esters of 14:0, 18:0 and cis 18:1 fatty acids all showed rs greater than 0.08 above the DSC melting point. The difference in rotational freedom of DPH in triacylglycerol and cholesteryl esters even in the 'liquid' state explains the low t0.08 in the more fluid plasma VLDL and the contrastingly high t0.08 in plasma LDL, and HDL, which contain more cholesteryl ester an less triacylglycerol.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
794
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-55
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Fluorescence polarization order parameters and phase transitions in lipids and lipoproteins.
pubmed:publicationType
Journal Article