Linked Life Data

673022

Source:http://linkedlifedata.com/resource/pubmed/id/673022

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1978-9-30
pubmed:abstractText
A sensitive radioimmunoassay for beta-endorphin is described. Antibodies against human beta-endorphin which exhibit a high avidity for the C-terminal of the peptide were raised in rabbits following the injection of thyroglobulin-coupled human beta-endorphin (betah-E) as immunogen. Methionine-enkephalin, alpha-, gamma-endorphine, as well as ACTH peptides did not cause interference in the radioimmunoassay. beta-Lipotropin, however, showed a 50% cross-reactivity. The sensitivity of the assay is 25 pg/0.5 ml tube volume for beta-endorphin. beta-Endorphin was extracted with a high recovery from the rat plasma using silicic acid and beta-endorphin levels as low as 100 pg/ml could be measured. Basal levels of beta-endorphin-like immunoreactivity in plasma of rats were about 400 pg/ml. beta-Endorphin levels in adrenalectomized rats and in animals chronically treated with the cortisol synthesis blocker metyrapone were found to be markedly increased (about 7-fold). Exposure of the rats to electrically induced footshocks caused a similar increase of immunoreactive beta-endorphin in plasma. A significant increase was also seen after insulin injection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0028-1298
pubmed:author
pubmed:issnType
Print
pubmed:volume
303
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-4
pubmed:dateRevised
2010-3-12
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Radioimmunoassay of beta-endorphin basal and stimulated levels in extracted rat plasma.
pubmed:publicationType
Journal Article