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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1984-7-9
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pubmed:abstractText |
An analytical method for the quantitation of nanogram to subnanogram amounts of dexamethasone is described. Dexamethasone was isolated from human plasma using a C18-bonded reverse-phase cartridge, purified by subsequent normal-phase HPLC, and the corresponding trimethylsilyl derivative analyzed by gas chromatography-mass spectrometry (GC-MS). The quantitation by isotope-dilution MS was carried out by selected-ion monitoring on the (M + 1)+ ion of the trimethylsilyl derivative of dexamethasone and its stable isotopically labeled diluent, [ 13C6 ,2H3]dexamethasone (681 and 690 m/z, respectively). Methane was used as the GC carrier gas and as the chemical-ionization reagent gas. The sensitivity of the method, judged from the lower limit of detection of the mass spectrometer, was at approximately 100 pg. The inter- and intraassay coefficients of variation (CV) determined at two different concentrations were 3.83 and 3.78% for 2 ng/mL and 2.64 and 1.29% for 5 ng/mL, respectively. Plasma concentration profiles for dexamethasone following a single 1-mg iv and a 2-mg oral dose of dexamethasone administered 24 h apart to two healthy volunteers are presented. The mass fragmentographic method described here is useful for bioavailability and pharmacokinetic studies of the synthetic glucocorticoid.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-3549
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
446-51
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:6726626-Administration, Oral,
pubmed-meshheading:6726626-Adult,
pubmed-meshheading:6726626-Biological Availability,
pubmed-meshheading:6726626-Chemical Phenomena,
pubmed-meshheading:6726626-Chemistry,
pubmed-meshheading:6726626-Dexamethasone,
pubmed-meshheading:6726626-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:6726626-Humans,
pubmed-meshheading:6726626-Injections, Intravenous,
pubmed-meshheading:6726626-Kinetics,
pubmed-meshheading:6726626-Male,
pubmed-meshheading:6726626-Mass Spectrometry,
pubmed-meshheading:6726626-Middle Aged,
pubmed-meshheading:6726626-Tablets
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pubmed:year |
1984
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pubmed:articleTitle |
Quantitative determination of dexamethasone in human plasma by stable isotope dilution mass spectrometry.
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pubmed:publicationType |
Journal Article
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