Linked Life Data

6721501

Source:http://linkedlifedata.com/resource/pubmed/id/6721501

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-6-14
pubmed:abstractText
Isolated rabbit liver branched-chain alpha-ketoacid dehydrogenase was inhibited in a mixed manner relative to ATP by alpha-ketoisocaproate, alpha-keto-beta-methylvalerate, alpha-ketoisovalerate, alpha-ketocaproate, alpha-ketovalerate, and alpha-chloroisocaproate with I40 values (mM), respectively, of 0.065, 0.49, 2.5, 0.2, 0.5, and 0.08. The concentration (mM) of alpha-ketoisocaproate, alpha-keto-beta-methylvalerate, and alpha-ketoisovalerate needed to activate branched-chain alpha-ketoacid dehydrogenase in the perfused rat heart to 50% of total activity was 0.07, 0.10, and 0.25, respectively. Isolated branched-chain alpha-ketoacid dehydrogenase kinase was inhibited (I40 values, mM) by octanoate (0.5), acetoacetyl-CoA (0.01), methylmalonyl-CoA (0.2), NADP+ (1.5), and heparin (12 micrograms/ml). The kinase activity, in the presence or absence of ADP, was inhibited approximately 30% by 0.1 mM isobutyryl-CoA, isovaleryl-CoA, and malonyl-CoA, while not affected by NAD+ and NADH (1 mM), CoA, acetyl-CoA, methylcrotonyl-CoA, crotonyl-CoA, beta-hydroxy-beta-methyl-glutaryl-CoA, octanoyl-CoA, succinyl-CoA, and propionyl-CoA (0.1 mM). The following compounds at 2 mM also did not inhibit branched-chain alpha-ketoacid dehydrogenase kinase; acetate, propionate, beta-hydroxybutyrate, lactate, acetoacetate, malonate, alpha-ketomalonate, succinate, citrate, oxaloacetate, FAD, and NADPH. These findings help explain the unique effects of Leu compared with Val and Ile on branched-chain amino acid metabolism and the differences between control of the kinases associated with pyruvate dehydrogenase and branched-chain alpha-ketoacid dehydrogenase.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
231
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
48-57
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Regulation of branched-chain alpha-ketoacid dehydrogenase kinase.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't