Linked Life Data

6714432

Source:http://linkedlifedata.com/resource/pubmed/id/6714432

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1984-6-14
pubmed:abstractText
When cultured on an impermeable substratum, the renal epithelial cell line Madin-Darby canine kidney (MDCK) forms domes. From a knowledge of dome shape, radius, and hydrostatic pressure difference delta P, from within to outside the domes, biophysical properties of the epithelium can be determined. By microscopy, circular based domes were found to be section of spheres with a constant height-to-radius ratio h/r of 0.684. By using the Laplace equation derived for this geometry and measurements of delta P and r, the values of the tension of cell dish adhesion Ta and dome wall tension Tw were found to be constants of 6.60 and 7.08 torr, respectively. By combining constants, Ta, and h/r, and because domes are section of spheres, delta P and dome volume were shown to be known functions of radius alone. The modulus of elasticity of the epithelium was calculated to be 4.82 X 10(3) dyn/cm2.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-9446
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2217-20
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Biophysics of domes formed by the renal cell line Madin-Darby canine kidney.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't