Linked Life Data

6713404

Source:http://linkedlifedata.com/resource/pubmed/id/6713404

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1984-6-1
pubmed:abstractText
A new method for autoradiographic measurement of unscheduled DNA synthesis (UDS) in the skin in vivo after treatment with ultraviolet light (UV) was developed. The skin of the back of ICR mice was shaved and exposed to short-wave UV (254 nm) or UVAB (sunlamp, 270 to 440 nm, predominant emission at 312 nm) at various doses. Immediately after irradiation, an isotonic aqueous solution of [methyl-3H]thymidine was injected s.c. into a portion of the skin clamped off with ring-shaped forceps. By this method, dose-dependent UDS was clearly demonstrated as silver grains on various types of cells in the skin in response to 254 nm UV or sunlamp UV. However, the energy values at the two wavelengths required to induce the same UDS level differed by 1 order of magnitude. These findings suggested that this system should be useful for quantitative analysis of UV-induced DNA repair in individual cells of the skin in vivo. By this method, the wavelength difference in transmissibility was studied. Autoradiographic results clearly showed that sunlamp UV could reach deeper sites in the skin than did 254 nm UV. A time course study indicated that UDS was almost complete by 48 hr after 254 nm UV but still persisted at 48 hr after sunlamp UV. These results, together with the differences in transmissibility, support higher tumorigenic activity of sunlamp UV than of 254 nm UV to experimental animals.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2150-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Dose response, wavelength dependence, and time course of ultraviolet radiation-induced unscheduled DNA synthesis in mouse skin in vivo.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't