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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1984-5-11
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pubmed:abstractText |
Heme-deficiency and double-stranded RNA (dsRNA) activate distinct cyclic 3':5'-AMP independent protein kinases (HRI and dsI, respectively) in rabbit reticulocyte lysates. These kinases inhibit protein synthesis by phosphorylating the 38,000 daltons (38K) subunit of the initiation factor eIF-2 (eIF-2 alpha). Using separation techniques to obtain a reticulocyte enriched fraction and reticulocyte-free erythrocytes, we have prepared lysates of these fractions from normal human whole blood. Human reticulocyte-enriched lysates contain the hemin-regulated and dsRNA-dependent protein kinases which inhibit protein synthesis and which phosphorylate rabbit eIF-2 alpha. An endogenous 38K polypeptide which co-migrates with rabbit eIF-2 alpha is also phosphorylated. In contrast, human mature erythrocytes contain little or no heme-regulated or dsRNA-dependent eIF-2 alpha kinase activities which are inhibitory of protein synthesis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
119
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
891-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:6712675-Blood Proteins,
pubmed-meshheading:6712675-Enzyme Activation,
pubmed-meshheading:6712675-Ethylmaleimide,
pubmed-meshheading:6712675-Eukaryotic Initiation Factor-2,
pubmed-meshheading:6712675-Heme,
pubmed-meshheading:6712675-Humans,
pubmed-meshheading:6712675-Kinetics,
pubmed-meshheading:6712675-Molecular Weight,
pubmed-meshheading:6712675-Peptide Initiation Factors,
pubmed-meshheading:6712675-Phosphorylation,
pubmed-meshheading:6712675-Protein Biosynthesis,
pubmed-meshheading:6712675-Protein Kinases,
pubmed-meshheading:6712675-RNA, Double-Stranded,
pubmed-meshheading:6712675-Reticulocytes,
pubmed-meshheading:6712675-eIF-2 Kinase
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pubmed:year |
1984
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pubmed:articleTitle |
Control of protein synthesis in human reticulocytes by heme-regulated and double-stranded RNA dependent eIF-2 alpha kinases.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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