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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1984-5-11
|
| pubmed:abstractText |
The fluorescence parameters of demetallized alpha-lactalbumin in the range from pH 8 to 2 show an extreme around pH 5-4 (a minimum in quantum yield and wavelength and a maximum in polarization). This extreme is not due to a competition between Ca2+ and protons but rather to a stabilization of the conformation of the protein near the isoelectric pH by the ionic interactions between local positive and negative charges on the protein. The calcium-free protein has similar fluorescence characteristics at pH 2 and 8 but the thermal transition curve is different. The influence of 0.1 M NaCl is also considered.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
119
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
509-15
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:6712641-Animals,
pubmed-meshheading:6712641-Calcium,
pubmed-meshheading:6712641-Cattle,
pubmed-meshheading:6712641-Hydrogen-Ion Concentration,
pubmed-meshheading:6712641-Kinetics,
pubmed-meshheading:6712641-Lactalbumin,
pubmed-meshheading:6712641-Spectrometry, Fluorescence,
pubmed-meshheading:6712641-Temperature
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
pH-dependence of the alpha-lactalbumin structure: a fluorescence study.
|
| pubmed:publicationType |
Journal Article
|