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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1984-5-23
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pubmed:abstractText |
Nanosecond laser flash photolysis has been used to produce and identify the vitamin K semiquinone (radical) from vitamin K dihydroquinone and to observe its formation and decay in the presence of vitamin K-dependent carboxylase (epoxidase). The activity of vitamin K-dependent carboxylase is not decreased by exposure to the laser. Absorbance of the semiquinone is proportional to enzyme concentration and is stimulated by a synthetic substrate, Phe-Leu-Glu-Glu-Ile. Stabilization of the semiquinone is observed in the presence of the enzyme. The semiquinone is rapidly destroyed in the presence of inhibitors of vitamin K-dependent carboxylase and vitamin K epoxidase.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
230
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
389-99
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6712248-Animals,
pubmed-meshheading:6712248-Carbon-Carbon Ligases,
pubmed-meshheading:6712248-Free Radicals,
pubmed-meshheading:6712248-Kinetics,
pubmed-meshheading:6712248-Lasers,
pubmed-meshheading:6712248-Ligases,
pubmed-meshheading:6712248-Male,
pubmed-meshheading:6712248-Photochemistry,
pubmed-meshheading:6712248-Rats,
pubmed-meshheading:6712248-Rats, Inbred Strains,
pubmed-meshheading:6712248-Vitamin K,
pubmed-meshheading:6712248-Vitamin K 1
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pubmed:year |
1984
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pubmed:articleTitle |
Vitamin K-dependent carboxylase: evidence for a semiquinone radical intermediate.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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