Linked Life Data

6712132

Source:http://linkedlifedata.com/resource/pubmed/id/6712132

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1984-5-11
pubmed:abstractText
We describe a simple, sensitive affinity technique for the routine measurement of glycosylated plasma proteins in clinical laboratories. The commercially available phenylboronic acid gel used for the chromatography has recently been marketed as a kit for this purpose (Glycogel Test Kit, Pierce Chemical Co). The manufacturers of this kit recommend loading 200 microliters neat plasma to each 1 ml gel column. This high loading is to enable the direct measurement of protein in the bound and unbound fractions at 280 nm. This loading is consistent with 10-15 mg protein being added per ml gel. Our results show that protein levels greater than 2 mg per ml gel overload the column. Therefore we used a modification of the more sensitive Bradford procedure to measure protein. The method discriminates between normals (6.29 +/- 1.87%) and diabetic patients (12.62 +/- 3.36%) and has good precision (CV 4-6%). The results obtained correlate with the colorimetric method using thiobarbituric acid (r = 0.70) and with glycosylated haemoglobin (r = 0.82).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0004-5632
pubmed:author
pubmed:issnType
Print
pubmed:volume
21 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16-21
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
A sensitive method for the measurement of glycosylated plasma proteins using affinity chromatography.
pubmed:publicationType
Journal Article