Linked Life Data

6708563

Source:http://linkedlifedata.com/resource/pubmed/id/6708563

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4A
pubmed:dateCreated
1984-5-18
pubmed:abstractText
The conversion of [14C]androstenedione by human fibroblast (F) and amniotic fluid (AF) cells obtained by amniocentesis was investigated using human dermal fibroblasts (DF) as controls. Cell suspensions were incubated with [14C]androstenedione in the presence of a NADPH generating system. Steroid reaction products were separated from unreacted substrate by chromatography on micro-columns of magnesium oxide, partially resolved by partition chromatography on celite and further characterized by thin-layer chromatography and recrystallization to constant specific activity. In the case of F cells the pattern of metabolism was qualitatively similar to that of DF cells; the predominant metabolite was testosterone and several uncharacterized metabolites were detectable. However testosterone was the only metabolite isolated from incubations with AF cells. The results demonstrate distinct differences in the capacity of AF and F type cells to metabolize [14C]androstenedione and support the view that F cells resemble typical fibroblasts from dermis or other connective tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-4731
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
935-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Metabolism of [4-14C]androstenedione by cells cultured from human amniotic fluid.
pubmed:publicationType
Journal Article