Linked Life Data

6705936

Source:http://linkedlifedata.com/resource/pubmed/id/6705936

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1984-5-14
pubmed:abstractText
Seven-hour and 20-hour incubation periods were tested with donor specimens to be used in the hamster egg sperm penetration assay (SPA). Aliquots of the washed sperm were incubated in air and in 5% CO2 and 95% air at 37 degrees C for comparison of sperm survival rates. The mean penetration for the donors ranged from 0% to 64%, with group means of 21% and 36% for the 7- and 20-hour incubations, respectively. All donors had penetration rates in the fertile range after 20 hours of incubation, but 33% (3 of 9) had penetration rates less than 10% after 7 hours of incubation. Incubation of sperm in air reduced the motility by 9% after 20 hours. The results suggest that the 20-hour incubation in 5% CO2 is better because it reduces the possibility of false-negative results in the SPA. We conclude that penetration in the questionable range of 10% may require further SPA testing with different incubation periods and technique modifications, such as a sperm rise step to increase sperm motility and reduce the number of leukocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0015-0282
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
599-602
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Hamster egg penetration: comparison of preincubation periods.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.