Linked Life Data

6704194

Source:http://linkedlifedata.com/resource/pubmed/id/6704194

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-4-17
pubmed:abstractText
Results of the solid phase F(ab')2 anti-C3 radioimmunoassay for immune complexes were abnormal in sera from some patients with juvenile arthritis and in many patients with other forms of chronic inflammation. However, C3 failed to block positive reactions of sera from patients even though it did block the reaction when aggregated IgG was added to normal sera. Moreover, positive sera reacted with nonspecific F(ab')2 fragments from several species. High correlation coefficients were obtained between abnormal results in the anti-C3 assay with juvenile arthritis sera and reactions with goat, mouse, and rabbit F(ab')2, but less so with human F(ab')2. Sera from patients with systemic lupus erythematosus or cystic fibrosis had similar reactions but different species specificities. After sucrose density fractionation, the reactivity with nonspecific anti-F(ab')2 was only in the IgG-containing fractions, but anti-C3 activity was in both IgG-containing fractions and the fractions between IgG and IgM. We concluded that anti-F(ab')2 antibodies are common in children with inflammation and may interfere with interpretation of assays using F(ab')2 anti-C3 for detection of complexes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0004-3591
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
330-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Anti-F(ab')2 antibodies that interfere with interpretation of the anti-C3 assay for immune complexes in children with rheumatic diseases.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't