Linked Life Data

6699456

Source:http://linkedlifedata.com/resource/pubmed/id/6699456

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-3-29
pubmed:abstractText
This is an initial descriptive report of two morphologically different cell lines (SKG-3a and SKG-3b) obtained from a human uterine cervical epidermoid carcinoma. These sublines were found to be derived from a moderately differentiated epidermoid carcinoma partially mixed with clear cell components. Both sublines had already been subcultivated more than 80 times since the initial separation. SKG-3a cells were much smaller in size and volume than 3b. The numbers of chromosomes were almost identical. Their cytology revealed anaplastic and pleomorphic features. SKG-3a was positive with periodic acid-Schiff stain and this changed to negative after an amylase digestive test, suggesting that the cells contained glycogen. An electron-microscopic examination confirmed the presence of many glycogen particles in 3a cytoplasm, but few in 3b. Tonofilaments and desmosomes were observed in both cytoplasms, suggesting epidermoid origin. In nude mice, SKG-3a produced clear cell epidermoid carcinoma with much glycogen, while 3b grew as a moderately differentiated epidermoid carcinoma with little glycogen. It is concluded that SKG-3a is derived from the clear cell epidermoid carcinoma and 3b is from the moderately differentiated epidermoid carcinoma. It is also clear that both were more heat sensitive at and over 39 degrees C than normal cells.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0300-9165
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-46
pubmed:dateRevised
2011-7-29
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
[Biological properties of two newly established cell lines (SKG-3a,3b) from a human uterine cervical epidermoid carcinoma].
pubmed:publicationType
Journal Article, English Abstract