Linked Life Data

6694041

Source:http://linkedlifedata.com/resource/pubmed/id/6694041

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-3-12
pubmed:abstractText
Turkey embryos were maintained in long-term, shell-less culture to the age of 17 days. At this time, 10 cultures were given saline (controls), and 10 cultures were given 30 micrograms zinc in saline (+Zn). In addition, all cultures received 5 microCi each of [35S]cystine and 65Zn dissolved in the saline and applied to the chorioallantoic membrane. After a further 20 h of incubation, no significant differences were found for embryo, liver, or yolk sac weights nor for relative liver size, hematocrit, serum protein, or serum concentrations of zinc and copper for the control and + Zn cultures. The addition of zinc to the cultures significantly (p less than 0.01) elevated hepatic and yolk sac cytosol 65Zn and zinc concentrations. More than 70% of the increased 65Zn and zinc in the cytosol of liver and yolk sac could be accounted for as that bound to a low-molecular-weight metallothionein (MT)-like protein. Added zinc significantly (p less than 0.01) increased the incorporation of [35S]cystine and 65Zn into the MT-like protein from liver and yolk sac indicating de novo synthesis of this protein. Liver and yolk sac MT-like proteins eluted from DEAE Sephadex A-25 columns as a single predominant species. The synthesis of an MT-like protein by liver and yolk sac suggests a role in embryonic zinc homeostasis. Moreover, shell-less culture of the avian embryo appears to be a useful method for studying zinc metabolism during periods of embryonic growth and development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0277-2116
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
128-36
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Synthesis of a metallothionein-like protein by developing turkey embryos maintained in long-term, shell-less culture.
pubmed:publicationType
Journal Article, In Vitro