Linked Life Data

6692828

Source:http://linkedlifedata.com/resource/pubmed/id/6692828

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-3-21
pubmed:abstractText
Carnitine acetyltransferase was synthesized in vitro in the mRNA-dependent reticulocyte system with mRNA from alkane-grown or propionate-grown cells of Candida tropicalis. The protein synthesized in vitro was isolated by immunoprecipitation with antibody against peroxisomal or mitochondrial carnitine acetyltransferase and was compared with peroxisomal carnitine acetyltransferase (Mr of subunits, 64 000 and 57 000) and the mitochondrial enzyme (Mr of subunits, 64 000 and 52 000) of C. tropicalis by electrophoresis in the presence of sodium dodecyl sulfate. Nascent carnitine acetyltransferase prepared in vitro showed a hetero-oligomeric property, like the peroxisomal and mitochondrial enzymes isolated from C. tropicalis. The molecular weights of the subunits of nascent carnitine acetyltransferase were estimated to be 71 000 and 57 000, indicating the existence of the precursor form of the enzyme. By sucrose density gradient centrifugation of total mRNA, these two subunit proteins were shown to be synthesized with respective mRNAs of different sizes. The same precursor-type of carnitine acetyltransferase was obtained with the mRNAs from the alkane-grown cells and the propionate-grown cells. The results obtained suggest that a common precursor will be post-translationally modified to form the peroxisomal and mitochondrial enzymes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
451-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Synthesis in vitro of precursor-type carnitine acetyltransferase with messenger RNA from Candida tropicalis.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't