Linked Life Data

6687461

Source:http://linkedlifedata.com/resource/pubmed/id/6687461

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-4-7
pubmed:abstractText
The apical regions of motile Limulus spermatozoa readily adhere to the outer layer of the egg envelope. Shortly after this adherence or primary attachment, the sperm acrosome reaction occurs, resulting in a stronger adhesion (secondary attachment). A sperm attachment assay that quantified the number of spermatozoa attaching to egg sections was utilized to identify components involved in primary attachment. The number of spermatozoa attached was examined after treatment of either egg sections or spermatozoa with various compounds. Egg sections treated with asparagus pea lectin (250 micrograms/ml) bound significantly fewer spermatozoa as compared to those exposed to wheat germ agglutinin, concanavalin A, and garden pea lectin. Furthermore, sperm attachment was also greatly reduced when egg sections were first incubated with the glycosidase, alpha-L-fucosidase (less than or equal to 5% of controls). Treatment of spermatozoa with alpha-L-fucose, fucoidan, or p-aminophenyl fucoside also reduced sperm attachment when compared to Millipore-filtered artificial seawater controls. Egg sections were treated with fluorescein-conjugated lectins to confirm that the lectins actually bound to portions of the egg envelope and that various sugars are present in the egg envelope. Evidence suggests that the methylpentose, alpha-L-fucose, plays an important role in primary sperm attachment in Limulus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
352-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Effect of lectins and sugars on primary sperm attachment in the horseshoe crab, Limulus polyphemus L.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't