Linked Life Data

6686086

Source:http://linkedlifedata.com/resource/pubmed/id/6686086

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 2
pubmed:dateCreated
1984-2-15
pubmed:abstractText
The differentiation of 3T3-F442A cells is accompanied by changes in cell morphology, decreased synthesis and assembly of cytoskeletal proteins, and increased biosynthesis of lipogenic enzymes. The differentiation-dependent alterations in actin assembly can be prevented by culture of cells on fibronectin matrices. Under these conditions, morphological change is prevented and the induction of lipogenic enzymes such as glycerophosphate dehydrogenase and fatty acid synthetase is sharply reduced. In contrast, the differentiation-linked decreases in actin and tubulin biosynthesis occur normally. Hybridization of cytoplasmic and nuclear RNA to cloned cDNA probes indicates that the inhibition of lipogenic protein biosynthesis occurs because of reduced levels of specific mRNA production. The inhibitory effects of fibronectin can be reversed by keeping cells in a rounded configuration or by exposing cells to either cytochalasin D, which disrupts the actin cytoskeleton, or insulin, a key lipogenic hormone. These results demonstrate that fibronectin can regulate gene expression for lipogenic proteins and they suggest that it interferes with cytoskeletal and morphological changes necessary for new gene expression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
657-66
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Fibronectin modulation of cell shape and lipogenic gene expression in 3T3-adipocytes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't