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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1984-1-27
|
| pubmed:abstractText |
An apparatus suitable for the recovery of proteins from polyacrylamide gels on a milligram scale by displacement electrophoresis (isotachophoresis) is described along with a buffer system that is suitable for this purpose with most proteins. The technique is illustrated by the recovery of a protein from a 15% polyacrylamide gel. The recovery was almost quantitative and the eluted protein showed little contamination upon quantitative amino acid analysis and automatic Edman degradation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
134
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
361-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:6685982-Amino Acid Sequence,
pubmed-meshheading:6685982-Chemical Phenomena,
pubmed-meshheading:6685982-Chemistry,
pubmed-meshheading:6685982-Electrophoresis,
pubmed-meshheading:6685982-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6685982-Humans,
pubmed-meshheading:6685982-Proteins,
pubmed-meshheading:6685982-Retinol-Binding Proteins
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Recovery of proteins on a milligram scale from polyacrylamide electrophoresis gels, exemplified by purification of a retinol-binding protein.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|