Linked Life Data

6668424

Source:http://linkedlifedata.com/resource/pubmed/id/6668424

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-3-30
pubmed:abstractText
The activity of NK cells in 12 healthy male donors was studied after 4, 8, 12, 16 and 20 h incubation of killer (mononuclear) cells with K562 target cells; a modification of the standard 3-5 h NK assay was used without preliminary centrifugation of the plates. The results indicated that 4 or 8 h incubation of killer and target cells (ratio 50:1) did not achieve maximum activity of NK cells. A significant increase in activity was found up to 12 h incubation. It was noted that variation in values decreased as the incubation time increased. The optimal incubation period was 16 h at which time maximum 51Cr release and least variation were registered. Longer incubation of K562 cells also contributed to their higher sensitivity to NK cells. The experimental data revealed in vitro factors responsible for fluctuations in the level of lytic NK activity in repeated assays in the same healthy donors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
321-6
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Factors responsible for fluctuations in human NK cell activity in vitro.
pubmed:publicationType
Journal Article