Linked Life Data

6654906

Source:http://linkedlifedata.com/resource/pubmed/id/6654906

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1984-2-14
pubmed:abstractText
Cultured porcine kidney cells (LLC-PK1) form polarized epithelia that transport glucose from apical to basal surface as in the renal proximal tube. The ability of these cells to transport glucose is known to increase as the epithelium forms and matures in culture. We find that epithelia grown in medium containing 25 mM glucose have reduced hexose transport compared to epithelia grown in 5 mM glucose. This difference is not the result of differences in seeding efficiency and can be reversed by changing the concentration of glucose in the growth medium. Increased transport in epithelia grown in 5 mM glucose is the result of increased influx on the sodium-coupled apical membrane transporter rather than changes in efflux. This difference is apparently the result of more apical membrane transporters in epithelia grown in 5 mM glucose. The number of high affinity phlorizin-binding sites is greater in epithelia grown in 5 mM glucose (about 0.8 pmol/10(6) cells) than in 25 mM glucose (about 0.25 pmol/10(6) cells). The increase in the number of glucose transporters induced by the low glucose medium is specific in that there is not a comparable change in activity of marker enzymes (alkaline phosphatase, acid phosphatase, or glucose 6-phosphatase). The nature of the intracellular signal elicited by extracellular glucose remains to be determined.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
258
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15087-90
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Regulation of sodium-coupled glucose transport by glucose in a cultured epithelium.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't