Linked Life Data

6652680

Source:http://linkedlifedata.com/resource/pubmed/id/6652680

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 2
pubmed:dateCreated
1984-2-15
pubmed:abstractText
The monoclonal antibody ECCD-1 inhibits Ca2+-dependent cell-cell adhesion in teratocarcinoma cells, recognizing a cell surface component of MW 124,000. When mouse embryos at various preimplantation stages were cultured in the presence of ECCD-1, the compacted morphology of the 8- to 16-cell-stage embryos was destroyed. In these embryos, cell proliferation normally occurred and development of blastocyst-like vesicles was attained. However, these embryos did not contain inner cell mass (ICM). We found that ECCD-1 affects the pattern of polarization of the cell surface in late 8- and 16-cell-stage blastomeres, as detected by staining with fluorescence-labeled concanavalin A. In normal blastomeres, the pole was always apart from the cell-cell contact plane. In those cultured in ECCD-1, formation of the pole tended to be inhibited, and if observed, the pole was close to the cell-cell contact plane. We discuss the possible mechanisms for inhibition of ICM formation caused by blocking Ca2+-dependent cell-cell adhesion between blastomeres.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
631-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
The calcium-dependent cell-cell adhesion system regulates inner cell mass formation and cell surface polarization in early mouse development.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't