Linked Life Data

6644388

Source:http://linkedlifedata.com/resource/pubmed/id/6644388

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1984-1-27
pubmed:abstractText
The role of Cu2+ on the DNA-breaking action of ascorbic acid (AsA) and triose reductone (TR) was studied with an agarose slab gel electrophoretic analysis. AsA and TR decomposed calf thymus DNA which had been pretreated with Cu2+, and their decomposing activity was proportional to the concentration of Cu2+ bound to the DNA. The DNA-Cu2+ complex had the ability to oxidize reductones. AsA and TR also decomposed the pretreated DNA with Cu2+ more markedly than that pretreated with Cu2+ and successively with EDTA. The DNA-breaking activity of AsA and TR showed no Cu2+-concentration dependency. The maximal fragmentation of DNA occurred at the concentration ratio of DNA and Cu2+ of 4:1. Excess concentration of Cu2+ decreased the activity of reductones. The present results indicate that the binding of Cu2+ to DNA molecules is also essential for the DNA-breaking action of AsA and TR in the presence of Cu2+ and that Cu2+ bound to DNA molecules has more effective promoting activity than free Cu2+.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0301-4800
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
489-95
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Function of Cu2+ on the DNA-breaking actions of ascorbic acid and triose reductone.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't